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Agilent 1290 Infinity I HPLC System w/ DAD
Wavelength Range (DAD) 190 to 640 nm
Flow Rate Range 0.001 to 5 mL/min
Pressure Range 0 to 1200 bar (0 to 120 MPa) at 2 mL/min
- Engineered for fast gradients, high resolution, and a wide variety of chromatography applications
- Advanced binary pump with ultra-low delay volumes and Jet Weaver mixing device
- Seamless method transfer compatibility with HPLC and UHPLC systems
- Variable volume autosampler with injection range from 0.1 to 20 uL and reduced carryover
- Thermostatted column compartment with a temperature range from 10°C below ambient to 100°C
- Max-Light cartridge cells with optofluidic waveguides for high sensitivity and low dispersion
- Diode-array detector features a data rate of up to 160 Hz
- Integrated degasser for enhanced flow stability and detector sensitivity
- Gradient formation with high-pressure binary mixing and solvent selection valves
- Supports both short and long columns with independent temperature zones
The Agilent 1290 Infinity I HPLC System with Diode-Array Detector (DAD) sets the standard for advanced liquid chromatography, offering unmatched precision, flexibility, and reliability. Designed to accommodate current and emerging column technologies, it ensures seamless method transfer from any HPLC or UHPLC system. With a wide flow rate range of 0.05 to 5 mL/min and a pressure capacity up to 1200 bar, it supports both conventional and ultra-high-performance chromatography. The advanced binary pump technology features heavy-duty motors, durable pistons for ultra-high pressure, and the innovative Jet Weaver mixing device, delivering ultra-low delay volumes and unparalleled flow stability. These features enable fast gradients, high resolution, and optimal compatibility with a variety of column diameters and mobile phase viscosities.
The system's variable volume autosampler is engineered for precision and efficiency, reducing carryover and ensuring accurate injections from 0.1 to 20 µL. Enhanced with advanced inert materials and automatic delay volume reduction (ADVR), it supports fast gradient delivery and reproducibility across the full injection range. The thermostatted column compartment further enhances performance, providing a temperature range from 10°C below ambient to 100°C with ±0.05°C stability. This compartment supports long and short columns, offers independent temperature zones, and ensures consistent results through a combination of pre-heating or cooling mobile phases, thermostat vanes, and still-air control.
The system’s DAD module leverages Max-Light cartridge cell technology with optofluidic waveguides to deliver high sensitivity, low dispersion, and a stable baseline. It features a wavelength range of 190 to 640 nm, a data collection rate of up to 160 Hz, and programmable optical slit adjustments for optimal spectral resolution and signal noise reduction. The Max-Light cartridge cells, available in standard 10 mm and optional 60 mm path lengths, are easily interchangeable and auto-aligned, ensuring consistent peak integration and sensitivity.
Additional system highlights include an integrated degasser for enhanced flow stability, a high-pressure binary gradient mixing capability, and solvent selection valves for flexible mobile phase management. The column compartment supports flow rates of up to 5 mL/min and accommodates three 30 cm columns, with rapid warm-up and cool-down times for increased efficiency. With its robust engineering, advanced features, and comprehensive operational range, the Agilent 1290 Infinity I HPLC System with DAD is the ultimate solution for laboratories requiring high-speed gradients, exceptional sensitivity, and reliable performance for demanding analytical applications.
The Agilent 1290 Infinity I HPLC System w/ DAD can be fully controlled from the Agilent ChemStation Version 4.3 Software, which allows for user-friendly operation in data acquisition, analysis, instrument control, automation, and more.
This Agilent system is in excellent condition and will be fully tested to perform at factory
specifications before being shipped.
The Agilent 1290 Infinity I HPLC System w/ DAD includes:
- > G4220A Binary Pump (With Integrated Degasser)
- > G4226A Autosampler
- > G1330B ALS Therm
- > G1316C Column Compartment
- > G4212A DAD Detector
- > Windows 11 Professional 64-Bit Data Station with Flatscreen Monitor
- > ChemStation Version 4.3 Software Installed
- > Can/Crossover Cables
- > Agilent Lan Interface Card Built-In
- > Solvent Tubing
- > 4 x Bottles, Caps and Solvent Filters
- > New Stainless Steel Capillaries
- > 1/4" Wrench & Mobile Phase Priming Syringe
- > Waste Tubing
- > All Power Cords
- > Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report
- > Full 180 Day Parts and Labor Warranty
Below is a List of Diagnostic Tests Performed On This System
- Filter Test: The test moves the filter into the light path and measures the relative absorbance in the filter position.
- Dark Current Test: The test measures the dark current from the detector optic.
- Holmium Oxide Test: The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
- Lamp Intensity Test: The test verifies functionality of the UV lamp at various wavelengths.
- Wavelength Intensity Test: The test confirms the accuracy of wavelengths measured.
- Baseline Noise Test: The test measures the amount of baseline noise drift identified by the detector.
- Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
- Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.
- Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.
- Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
- 4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the Agilent 1260 Infinity II LC System w/ MWD.
Agilent 1290 Infinity I HPLC System w/ DAD Specifications
DAD Detector
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Specification
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Detection Type
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1024-element photodiode array
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Light Source
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Deuterium lamp
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Wavelength Range
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190 to 640 nm
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Short Term Noise
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With 10 mm Max-Light cartridge cell: < ± 3 x 10
-6 AU at 230/4 nm (included)
With 60 mm Max-Light cartridge cell: < ± 0.6 x 10
-6 AU at 230/4 nm
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Drift
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< 0.5 x 10
-3 AU/h at 230 nm
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Linear Absorbance Range
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> 2.0 AU (5%) at 265 nm
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Wavelength Accuracy
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± 1 nm
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Wavelength Bunching
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2 to 400 nm
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Diode Width
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~ 0.5 nm
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Signal Data Rate
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Up to 160 Hz
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Spectra Data Rate
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Up to 160 Hz
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Flow Cell
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10 mm Max-Light cartridge cell
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Click Here to View More Specifications for the Agilent 1290 Infinity I HPLC System w/ DAD
Binary Pump
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Specification
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Settable Flow Range
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0.001 to 5.0 mL/min, in 0.001 mL/min increments
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Flow Precision
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≤ 0.07 % RSD or 0.005 min SD, whatever is greater (0.2 to 5.0 mL/min)
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Flow Accuracy
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± 1 % or 10 uL/min, whichever is greater Pumping degassed H2O at 10 MPa (100 bar)
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Pressure Operating Range
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Up to 120 MPa (1200 bar) at up to 2 mL/min; 80 MPa (800 bar) at 5 mL/min
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Pressure Pulsation
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< 1 % amplitude or < 0.5 MPa (5 bar), whatever is greater
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Compressibility Compensation
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Automatic, pre-defined, based on mobile phase selection
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Gradient Formation
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High-pressure binary mixing
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Delay Volume
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Jet Weaver V35: < 45 uL
Jet Weaver V100: < 75 uL
For lowest delay volumes, the Jet Weaver can be removed from flow path
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Composition Range
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Settable range: 0 to 100 %
Recommended range: 1 to 99% or 5 uL/min per channel, whatever is greater
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Composition Precision
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< 0.15 % RSD or 0.01 min SD, whatever is greater
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Composition Accuracy
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± 0.35 % absolute (5 to 95 %, 0.2 to 5.0 mL/min)
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Solvent Selection Valve
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Default
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Integrated Degassing Unit
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Number of channels: 2; Internal volume per channel: 1.5 mL
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Autosampler
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Specification
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Injection Range
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0.1 - 20 uL in 0.1 uL increments
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Precision
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Typically < 0.25% RSD from 5 to 40 uL
Typically < 0.5% RSD from 2 to 5 uL Typically < 0.7% from 1 to 2 uL
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Pressure Range
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Up to 600 bar
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Sample Viscosity Range
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0.2 - 5 cp
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Sample Capacity
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2 x well plates (MTP) + 10 x 2 mL vials
108 x 2 mL vials in 2 x 54 vial plate plus 10 additional 2 mL vials (included) 30 x 6 mL vials in 2 x 15 vial plate 100 Micro vial tray plus 10 additional 2 mL vials 54 Eppendorf tubes (0.5/1.5/2 mL) in 2 x 27 Eppendorf tube plates
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Injection Cycle Time
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Typically < 21 seconds using draw speed of 100 uL/min, eject speed of 100 uL/min, and injection volume of 5 uL
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Carryover
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Typically < 0.004%
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ALS Thermostat
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Specification
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Temperature Range
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Settable from 4°C to 40°C in 1°C increments
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Temperature Accuracy at Ambient Temperatures < 25°C and Humidity < 50%
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3°C to 8°C at a setpoint of 4°C
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Temperature Accuracy at Ambient Temperatures > 30°C and Humidity > 60%
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3°C to 9°C at a setpoint of 4°C
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Column Compartment
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Specification
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Temperature Range
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Ambient -10°C to 80°C at flow rates up to 5 mL/min (up to 100°C at flow rates up to 2.5 mL/min
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Temperature Stability
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± 0.05°C
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Temperature Accuracy
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± 0.8°C
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Column Capacity
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Three 30 cm columns
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Warm-Up/Cool-Down Time
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5 minutes from ambient to 40°C
10 minutes from 40°C to 20°C
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Dead Volume
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1.6 uL low dispersion heat exchanger
3 uL left heat exchanger
6 uL right heat exchanger
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Computer Specifications
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Processor
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Intel Core I5-8500 CPU
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RAM
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32.0 GB
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Hard Drive
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500GB Internal SSD
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Operating System
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Windows 11 Professional, 64-bit
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- Verify uniformity of main and residential firmware across all components, updating as necessary to ensure compatibility
- Confirm successful communication between software and the HPLC system, validating data transfer integrity
- Check the degasser’s internal vacuum to confirm it reaches the specified vacuum level for optimal performance
- Replace inlet, outlet, purge valve, and pump seals in the system pump to maintain reliable operation
- Ensure system pump passes pressure and leak tests, confirming secure and efficient performance
- Replace all capillary tubing with new OEM stainless steel tubing for durability and precision
- Remove autosampler transport assembly, replace X and Y bearings, and thoroughly clean carrier rails to remove debris and contaminants
- Replace metering valve seal, autosampler needle, and needle seat for consistent sampling accuracy
- Test column compartment switching valve functionality and replace its seal if necessary
- Confirm column compartment passes thermostat test to maintain precise temperature control
- Remove and clean internal detector optics for clear and accurate signal detection
- Replace UV and Vis detector lamps to ensure optimal light source stability and performance
- Verify flow cell absorbance meets manufacturer’s specifications for accurate measurement
- Confirm system response to isocratic test sample, ensuring stable and reproducible results
Technician Inspection and Recertification Checklist: HPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
Task
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Completed?
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Remarks?
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Verify firmware uniformity and update as necessary
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Completed
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Confirm software communication with HPLC system
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Completed
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Check degasser vacuum level for optimal performance
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Completed
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Replace inlet, outlet, purge valve, and pump seals
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Completed
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Ensure pump passes pressure and leak tests
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Completed
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Replace all capillary tubing with OEM stainless steel tubing
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Completed
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Remove autosampler transport assembly, replace bearings, and clean rails
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Completed
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Replace metering valve seal, autosampler needle, and needle seat
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Completed
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Test column compartment switching valve and replace seal if needed
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Completed
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Confirm thermostat test in column compartment
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Completed
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Clean internal detector optics
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Completed
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Replace UV and Vis detector lamps
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Completed
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Verify flow cell absorbance meets manufacturer’s specifications
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Completed
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Confirm system response to isocratic test sample
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Completed
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To View More of Our Recertification Protocol Click Here
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