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New!  Agilent 1290 Infinity I HPLC System w/ DAD
Agilent 1290 Infinity I HPLC System w/ DAD
 
 
Our Price: $41,995.00

Condition: Fully Recertified and Tested to Perform at Manufacturer Specifications
Warranty: 180-Day Full Parts and Labor (Click for Info)
Typically Ships Within: 3 to 5 Business Days
Included Documentation Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report

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Product Code: AG-1290HPLCDAD
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Description
Recertification Protocol
Technical Specification Sheet
 

Agilent 1290 Infinity I HPLC System w/ DAD



Wavelength Range (DAD)
190 to 640 nm

Flow Rate Range
0.001 to 5 mL/min

Pressure Range
0 to 1200 bar (0 to 120 MPa) at 2 mL/min

Features
  • Engineered for fast gradients, high resolution, and a wide variety of chromatography applications
  • Advanced binary pump with ultra-low delay volumes and Jet Weaver mixing device
  • Seamless method transfer compatibility with HPLC and UHPLC systems
  • Variable volume autosampler with injection range from 0.1 to 20 uL and reduced carryover
  • Thermostatted column compartment with a temperature range from 10°C below ambient to 100°C
  • Max-Light cartridge cells with optofluidic waveguides for high sensitivity and low dispersion
  • Diode-array detector features a data rate of up to 160 Hz
  • Integrated degasser for enhanced flow stability and detector sensitivity
  • Gradient formation with high-pressure binary mixing and solvent selection valves
  • Supports both short and long columns with independent temperature zones

The Agilent 1290 Infinity I HPLC System with Diode-Array Detector (DAD) sets the standard for advanced liquid chromatography, offering unmatched precision, flexibility, and reliability. Designed to accommodate current and emerging column technologies, it ensures seamless method transfer from any HPLC or UHPLC system. With a wide flow rate range of 0.05 to 5 mL/min and a pressure capacity up to 1200 bar, it supports both conventional and ultra-high-performance chromatography. The advanced binary pump technology features heavy-duty motors, durable pistons for ultra-high pressure, and the innovative Jet Weaver mixing device, delivering ultra-low delay volumes and unparalleled flow stability. These features enable fast gradients, high resolution, and optimal compatibility with a variety of column diameters and mobile phase viscosities.

The system's variable volume autosampler is engineered for precision and efficiency, reducing carryover and ensuring accurate injections from 0.1 to 20 µL. Enhanced with advanced inert materials and automatic delay volume reduction (ADVR), it supports fast gradient delivery and reproducibility across the full injection range. The thermostatted column compartment further enhances performance, providing a temperature range from 10°C below ambient to 100°C with ±0.05°C stability. This compartment supports long and short columns, offers independent temperature zones, and ensures consistent results through a combination of pre-heating or cooling mobile phases, thermostat vanes, and still-air control.

The system’s DAD module leverages Max-Light cartridge cell technology with optofluidic waveguides to deliver high sensitivity, low dispersion, and a stable baseline. It features a wavelength range of 190 to 640 nm, a data collection rate of up to 160 Hz, and programmable optical slit adjustments for optimal spectral resolution and signal noise reduction. The Max-Light cartridge cells, available in standard 10 mm and optional 60 mm path lengths, are easily interchangeable and auto-aligned, ensuring consistent peak integration and sensitivity.

Additional system highlights include an integrated degasser for enhanced flow stability, a high-pressure binary gradient mixing capability, and solvent selection valves for flexible mobile phase management. The column compartment supports flow rates of up to 5 mL/min and accommodates three 30 cm columns, with rapid warm-up and cool-down times for increased efficiency. With its robust engineering, advanced features, and comprehensive operational range, the Agilent 1290 Infinity I HPLC System with DAD is the ultimate solution for laboratories requiring high-speed gradients, exceptional sensitivity, and reliable performance for demanding analytical applications.

The Agilent 1290 Infinity I HPLC System w/ DAD can be fully controlled from the Agilent ChemStation Version 4.3 Software, which allows for user-friendly operation in data acquisition, analysis, instrument control, automation, and more.

This Agilent system is in excellent condition and will be fully tested to perform at factory specifications before being shipped.

INCLUDED:
The Agilent 1290 Infinity I HPLC System w/ DAD includes:
  • > G4220A Binary Pump (With Integrated Degasser)
  • > G4226A Autosampler
  • > G1330B ALS Therm
  • > G1316C Column Compartment
  • > G4212A DAD Detector
  • > Windows 11 Professional 64-Bit Data Station with Flatscreen Monitor
  • > ChemStation Version 4.3 Software Installed
  • > Can/Crossover Cables
  • > Agilent Lan Interface Card Built-In
  • > Solvent Tubing
  • > 4 x Bottles, Caps and Solvent Filters
  • > New Stainless Steel Capillaries
  • > 1/4" Wrench & Mobile Phase Priming Syringe
  • > Waste Tubing
  • > All Power Cords
  • > Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report
  • > Full 180 Day Parts and Labor Warranty


Below is a List of Diagnostic Tests Performed On This System
G4212A Diode Array Detector Diagnostic Tests:
  • Filter Test: The test moves the filter into the light path and measures the relative absorbance in the filter position.
  • Dark Current Test: The test measures the dark current from the detector optic.
  • Holmium Oxide Test: The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
  • Lamp Intensity Test: The test verifies functionality of the UV lamp at various wavelengths.
  • Wavelength Intensity Test: The test confirms the accuracy of wavelengths measured.
  • Baseline Noise Test: The test measures the amount of baseline noise drift identified by the detector.
G4220A Binary Pump Diagnostic Tests:
  • Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
  • Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.
G1316C Column Compartment Diagnostic Tests:
  • Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.
G4226A Autosampler Diagnostic Tests:
  • Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
System Tests:
  • 4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the Agilent 1260 Infinity II LC System w/ MWD.

Agilent 1290 Infinity I HPLC System w/ DAD Specifications

DAD Detector Specification

Detection Type

1024-element photodiode array
Light Source Deuterium lamp
Wavelength Range 190 to 640 nm
Short Term Noise

With 10 mm Max-Light cartridge cell: < ± 3 x 10 -6 AU at 230/4 nm (included)

With 60 mm Max-Light cartridge cell: < ± 0.6 x 10 -6 AU at 230/4 nm

Drift < 0.5 x 10 -3 AU/h at 230 nm
Linear Absorbance Range > 2.0 AU (5%) at 265 nm
Wavelength Accuracy ± 1 nm
Wavelength Bunching 2 to 400 nm
Diode Width ~ 0.5 nm
Signal Data Rate Up to 160 Hz
Spectra Data Rate Up to 160 Hz
Flow Cell 10 mm Max-Light cartridge cell

Click Here to View More Specifications for the Agilent 1290 Infinity I HPLC System w/ DAD

Binary Pump Specification
Settable Flow Range 0.001 to 5.0 mL/min, in 0.001 mL/min increments
Flow Precision ≤ 0.07 % RSD or 0.005 min SD, whatever is greater (0.2 to 5.0 mL/min)
Flow Accuracy ± 1 % or 10 uL/min, whichever is greater Pumping degassed H2O at 10 MPa (100 bar)
Pressure Operating Range Up to 120 MPa (1200 bar) at up to 2 mL/min; 80 MPa (800 bar) at 5 mL/min
Pressure Pulsation < 1 % amplitude or < 0.5 MPa (5 bar), whatever is greater
Compressibility Compensation Automatic, pre-defined, based on mobile phase selection
Gradient Formation High-pressure binary mixing
Delay Volume

Jet Weaver V35: < 45 uL

Jet Weaver V100: < 75 uL

For lowest delay volumes, the Jet Weaver can be removed from flow path

Composition Range

Settable range: 0 to 100 %

Recommended range: 1 to 99% or 5 uL/min per channel, whatever is greater

Composition Precision < 0.15 % RSD or 0.01 min SD, whatever is greater
Composition Accuracy ± 0.35 % absolute (5 to 95 %, 0.2 to 5.0 mL/min)
Solvent Selection Valve Default
Integrated Degassing Unit Number of channels: 2; Internal volume per channel: 1.5 mL
Autosampler Specification
Injection Range 0.1 - 20 uL in 0.1 uL increments
Precision Typically < 0.25% RSD from 5 to 40 uL
Typically < 0.5% RSD from 2 to 5 uL
Typically < 0.7% from 1 to 2 uL
Pressure Range Up to 600 bar
Sample Viscosity Range 0.2 - 5 cp
Sample Capacity 2 x well plates (MTP) + 10 x 2 mL vials
108 x 2 mL vials in 2 x 54 vial plate plus 10 additional 2 mL vials (included)
30 x 6 mL vials in 2 x 15 vial plate
100 Micro vial tray plus 10 additional 2 mL vials
54 Eppendorf tubes (0.5/1.5/2 mL) in 2 x 27 Eppendorf tube plates
Injection Cycle Time Typically < 21 seconds using draw speed of 100 uL/min, eject speed of 100 uL/min, and injection volume of 5 uL
Carryover Typically < 0.004%
ALS Thermostat Specification
Temperature Range Settable from 4°C to 40°C in 1°C increments
Temperature Accuracy at Ambient Temperatures < 25°C and Humidity < 50% 3°C to 8°C at a setpoint of 4°C
Temperature Accuracy at Ambient Temperatures > 30°C and Humidity > 60% 3°C to 9°C at a setpoint of 4°C
Column Compartment Specification
Temperature Range Ambient -10°C to 80°C at flow rates up to 5 mL/min (up to 100°C at flow rates up to 2.5 mL/min
Temperature Stability ± 0.05°C
Temperature Accuracy ± 0.8°C
Column Capacity Three 30 cm columns
Warm-Up/Cool-Down Time 5 minutes from ambient to 40°C
10 minutes from 40°C to 20°C
Dead Volume

1.6 uL low dispersion heat exchanger

3 uL left heat exchanger

6 uL right heat exchanger



Computer Specifications

Processor Intel Core I5-8500 CPU
RAM 32.0 GB
Hard Drive 500GB Internal SSD
Operating System Windows 11 Professional, 64-bit
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