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Agilent 1260 Infinity II LC System w/ Vialsampler & DAD
Wavelength Range (DAD) 190 to 950 nm
Flow Rate Range 0.001 to 5.0 ml/min
Pressure Range Up to 60 MPa (600 bar, 8700 psi) up to 5 ml/min
- Provides liquid chromatograph offering ultra fast and high resolution separation capability for consistently accurate results
- Delivers several ease-of-use features, making the 1260 Infinity II a standard system for routine analysis
- Binary Pump ensures reproducible gradients and high-performance, ideal for routine applications with UV or MS detection (Quaternary Pump available, please inquire)
- Integrated degasser in the Binary Pump with a volume of 0.45ml per channel helps maintain system stability and performance
- Diode Array Detector (DAD) enhances light transmission to near 100% efficiency, reducing baseline drift and improving peak integration
- Minimal carryover with the Vialsampler, ensuring high reliability for routine pharmaceutical, environmental, and food analyses
- Fully controllable via Agilent OpenLab CDS Software for seamless operation in data acquisition, analysis, and instrument control
- Allows for user configuration of standard or low delay volumes, tailoring the system to specific analytical needs
- Equipped with spectral tools for comprehensive data analysis, including spectral libraries and peak purity functions
The Agilent 1260 Infinity II LC System w/ Vialsampler & DAD provides a liquid chromatograph offering ultra fast and high resolution separation capability for consistently accurate results. The 1260 Infinity II LC System delivers several ease-of-use features, making it a standard system for routine analysis. With an operating pressure of up to 600 bar and flow range from 0.001 to 5.0 ml/min, the 1260 Infinity II Binary Pump is suited for LC applications using conventional or superficially porous particle columns, as well as STM column technology. Gradient formation is based on a high-pressure mixing principle. Standard or low delay volumes can be easily configured by the user. The Agilent 1260 Infinity II Binary Pump offers reproducible gradients and high-performance, providing high-throughput and fast separations. The pump is suitable for routine applications, with UV or MS detection, where high speed and resolution with uncompromised data quality are required. A two-channel integrated degasser is located inside of the pump, which allows for a volume of 0.45ml per channel.
The Agilent 1260 Infinity II Vialsampler is an autosampler designed for the reliability and ease-of-use needed for routine pharmaceutical tasks and quality control, as well as for environmental and food analyses. It houses an integrated column oven for two LC columns up to 30 mm in length. The integrated column oven paired with a sample cooler allows for temperature control from 4°C to 80°C, all within one module. Two classic trays are included with the Vialsampler, which allows for a capacity of up to 100 x 2 mL vials. The Vialsampler allows for an injection range of 0.1 to 900 µl up to a pressure of 40 MPa.
The Agilent 1290 Infinity II Diode Array Detector (DAD) is based on the Agilent Max-Light cartridge cell with optofluidic waveguides that improve light transmission to near 100% efficiency without sacrificing resolution caused by cell dispersions effects. With typical detector noise levels of ± 0.6 x 10 -6 AU the 60 mm flow cell gives up to 10 times higher sensitivity than detectors with conventional flow cells. Any compromising refractive index and thermal effects are almost eliminated entirely, resulting in significantly less baseline drift for more reliable and precise peak integration. For fast separations, this detector has multiple wavelength and full spectral detection at sampling rates up to 240 Hz. The long-life deuterium lamp allows for the highest intensity and lowest detection limits over a wavelength range of 190 to 950 nm.
The Agilent 1260 Infinity II LC System w/ Vialsampler & DAD can be fully controlled from the Agilent OpenLab CDS Software, which allows for user-friendly operation in data acquisition, analysis, instrument control, automation, and more.
This Agilent system is in excellent condition and will be fully tested to perform at factory
specifications before being shipped.
The Agilent 1260 Infinity II LC System w/ Vialsampler & DAD includes:
- > G7112B Binary Pump (With Integrated Degasser) [G7111B Quaternary Pump Upgrade Available, Please Inquire]
- > G7115A DAD Detector
- > G7129A Vialsampler (With Integrated Column Oven)
- > Windows 11 Professional 64-Bit Data Station with Flatscreen Monitor
- > OpenLab CDS Software Installed
- > Can/Crossover Cables
- > Agilent Lan Interface Card Built-In
- > Solvent Tubing
- > 4 x Bottles, Caps and Solvent Filters
- > New Stainless Steel Capillaries
- > 1/4" Wrench & Mobile Phase Priming Syringe
- > Waste Tubing
- > All Power Cords
- > Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report
- > Full 180 Day Parts and Labor Warranty
Below is a List of Diagnostic Tests Performed On This System
- Filter Test: The test moves the filter into the light path and measures the relative absorbance in the filter position.
- Dark Current Test: The test measures the dark current from the detector optic.
- Holmium Oxide Test: The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
- Lamp Intensity Test: The test verifies functionality of the UV lamp at various wavelengths.
- Wavelength Intensity Test: The test confirms the accuracy of wavelengths measured.
- Baseline Noise Test: The test measures the amount of baseline noise drift identified by the detector.
- Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
- Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.
- Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.
- Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
- 4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the Agilent 1260 Infinity II LC System w/ Vialsampler & DAD.
Agilent 1260 Infinity II LC System w/ Vialsampler & DAD Specifications
DAD Detector
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Specification
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Detection Type
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1024-element photodiode array
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Light Source
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Deuterium lamp
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Number of Signals
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8
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Maximum Sampling Rate
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240 Hz (both spectra and signals)
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Wavelength Range
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190 - 950 nm
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Short Term Noise
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With 10 mm Max-Light cartridge cell: < ± 3 x 10
-6 AU at 230/4 nm, slit width 4 nm, TC 2 s , ASTM
With 60 mm Max-Light cartridge cell (included): < ± 0.6 x 10
-6 AU at 230/4 nm, slit width 4 nm, TC 2 s , ASTM
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Drift
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< 0.5 x 10
-3 AU/h at 230 nm
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Linearity
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> 2.0 AU (5%) at 265 nm, typically 2.5 AU (5%)
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Wavelength Accuracy
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± 1 nm
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Wavelength Bunching
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2 - 400 nm in steps of 1 nm
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Wavelength Precision
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< ± 0.1 nm
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Slit Width
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1, 2, 4, 8 nm
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Diode Width
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~0.5 nm
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Flow Cell
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Max-Light cartridge cell (high sensitivity): 60 mm
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Spectral Tools
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Data analysis software for spectra evaluation, including spectral libraries and peak purity functions
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Click Here to View More Specifications for the Agilent 1260 Infinity II LC System w/ Vialsampler & DAD
Binary Pump
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Specification
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Hydraulic System
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Two dual piston in series pumps with servo-controlled variable stroke drive, power transmission by gears and ball screws, floating pistons
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Settable Flow Range
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0.001 - 5.0 ml/min, in 0.001 ml/min increments
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Recommended Flow Range
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0.05 - 5.0 ml/min
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Flow Precision
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≤ 0.07% RSD or ≤ 0.02 min SD, whichever is greater
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Flow Accuracy
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± 1% or 10 µl/min, whichever is greater, pumping degassed H2O at 10 MPa (100 bar)
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Pressure Operating Range
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Up to 60 MPa (600 bar, 8700 psi) up to 5 ml/min
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Pressure Pulsation
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< 2% amplitude (typically < 1.3%) or < 0.3 MPa (3 bar, 44 psi), whichever is greater
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Compressibility Compensation
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Pre-defined, based on mobile phase compressibility
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Recommended pH Range
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1.0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
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Gradient Formation
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High-pressure binary mixing
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Delay Volume
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600 - 900 µl, dependent on back pressure; measured with water at 1 ml/min (water/caffeine tracer)
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Settable Composition Range
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0 - 100%
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Recommended Compostion Range
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1 - 99% or 5µl/min per channel, whichever is greater
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Composition Precision
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< 0.15% RSD or < 0.04 min SD, whatever is greater
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Integrated Degassing Unit
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Number of channels: 2
Internal volume per channel: 0.45 ml
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Vialsampler
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Specification
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Injection Range
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0.1 - 900 µl in 0.1 µl increments with 900 µl up to 40 MPa
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Injection Precision
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< 0.25% RSD of peak areas from 5 µl to 100 µl
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Pressure Range
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0 - 40 MPa (0 - 400 bar, 0 - 5801 psi)
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Sample Viscosity Range
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0.2 - 5 cp
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Sample Capacity
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100 x 2 mL vial (two classic trays included)
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Injection Cycle Time
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18 s for draw speed 200 µl/min
Ejection speed: 200 µl/min
Injection volume: 1 µl
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Carry Over
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1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial.
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Integrated Column Oven
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Specification
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Temperature Range
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Ambient +4°C to 80°C
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Temperature Accuracy
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2°C - 6°C at a setpoint of 4°C
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Column Capacity
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2 columns of up to 30 mm length
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- Verify uniformity of main and residential firmware across all components, updating as necessary to ensure compatibility
- Confirm successful communication between software and the HPLC system, validating data transfer integrity
- Check the degasser’s internal vacuum to confirm it reaches the specified vacuum level for optimal performance
- Replace inlet, outlet, purge valve, and pump seals in the system pump to maintain reliable operation
- Ensure system pump passes pressure and leak tests, confirming secure and efficient performance
- Replace all capillary tubing with new OEM stainless steel tubing for durability and precision
- Remove autosampler transport assembly, replace X and Y bearings, and thoroughly clean carrier rails to remove debris and contaminants
- Replace metering valve seal, autosampler needle, and needle seat for consistent sampling accuracy
- Test column compartment switching valve functionality and replace its seal if necessary
- Confirm column compartment passes thermostat test to maintain precise temperature control
- Remove and clean internal detector optics for clear and accurate signal detection
- Replace UV and Vis detector lamps to ensure optimal light source stability and performance
- Verify flow cell absorbance meets manufacturer’s specifications for accurate measurement
- Confirm system response to isocratic test sample, ensuring stable and reproducible results
Technician Inspection and Recertification Checklist: HPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
Task
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Completed?
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Remarks?
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Verify firmware uniformity and update as necessary
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Completed
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Confirm software communication with HPLC system
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Completed
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Check degasser vacuum level for optimal performance
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Completed
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Replace inlet, outlet, purge valve, and pump seals
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Completed
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Ensure pump passes pressure and leak tests
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Completed
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Replace all capillary tubing with OEM stainless steel tubing
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Completed
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Remove autosampler transport assembly, replace bearings, and clean rails
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Completed
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Replace metering valve seal, autosampler needle, and needle seat
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Completed
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Test column compartment switching valve and replace seal if needed
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Completed
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Confirm thermostat test in column compartment
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Completed
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Clean internal detector optics
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Completed
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Replace UV and Vis detector lamps
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Completed
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Verify flow cell absorbance meets manufacturer’s specifications
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Completed
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Confirm system response to isocratic test sample
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Completed
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To View More of Our Recertification Protocol Click Here
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