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Agilent 1260 Infinity II LC System w/ VWD & RID
The Agilent 1260 Infinity II LC System w/ VWD & RID provides a liquid chromatograph offering ultra fast and high resolution separation capability for consistently accurate results. The 1260 Infinity II LC System delivers several ease-of-use features, making it a standard system for routine analysis. With an operating pressure of up to 600 bar the 1260 Infinity II Quaternary Pump is compatible with HPLC and UHPLC, i.e. handling 2.1, 3, and 4.6 mm ID columns over the flow rate range (up to 5 mL/min) and semi preparative analysis due to flow up to 10 mL/min. The 1260 Infinity II Multisampler can handle both vials and microtiter plates with ease and efficiency, and is designed for low carryover, as you can take clean to a whole new level with the multi-wash capability, cleaning all relevant injection parts between runs. This integrated feature flushes the injection needle outside with three solvents, and uses seat back flush procedures to reduce carryover to less than 9 ppm.
The Agilent 1260 Infinity II Variable Wavelength Detector (VWD) is the most sensitive and fastest detector in its class. The detector features time-programmable wavelength switching provides sensitivity and selectivity for your applications. More sample information can be acquired in the dual wavelength mode. Low detector noise (< ± 2.5 AU) and baseline drift (< 1 x 10^-4 AU/h) facilitates precise quantification of trace levels components. High productivity can be achieved with fast analysis at up to 120 Hz data rates. The Agilent 1260 Infinity II Refractive Index Detector (RID) is the ideal detector for fast and reliable LC results when routinely analyzing non-UV absorbing substances, such as carbohydrates, lipids, and polymers. The 1260 Infinity II RID is also the detector of choice for gel permeation chromatography (GPC) or size exclusion chromatography (SEC). Some of the features this RID detector includes are: high sensitivity and robust detection with an excellent reproducibility of only 0.1 % RSD, precise temperature control from ambient +5 °C to 55 °C to minimize baseline drift and optimize compound response, automatic light level adjustment compensates for lamp degradation and flow cell staining, ensuring optimum performance and lowest maintenance, and more. The Agilent 1260 Infinity II Multicolumn Thermostat (MCT) allows precise column thermostatting over a broad temperature range: from cooling down to 10 degrees below ambient temperature up to 85°C, providing high flexibility for optimized speed and selectivity of LC separation. For bio-inert applications bio-inert heat exchangers and a selection of bio-inert valves are offered by Agilent.
The Agilent 1260 Infinity II LC System w/ VWD & RID can be fully controlled from the Agilent OpenLab CDS Software, which allows for user-friendly operation in data acquisition, analysis, instrument control, automation, and more.
This Agilent system is in excellent condition and will be fully tested to perform at factory
specifications before being shipped.
The Agilent 1260 Infinity II LC System w/ VWD & RID includes:
- > G7111B Quaternary Pump (With Integrated Degasser)
- > G7114A VWD Detector
- > G7162A RID Detector
- > G7167A Multisampler
- > G7116A Multicolumn Thermostat
- > Windows 11 Professional 64-Bit Data Station with Flatscreen Monitor
- > OpenLab CDS Software Installed
- > Can/Crossover Cables
- > Agilent Lan Interface Card Built-In
- > Solvent Tubing
- > 4 x Bottles, Caps and Solvent Filters
- > New Stainless Steel Capillaries
- > 1/4" Wrench & Mobile Phase Priming Syringe
- > Waste Tubing
- > All Power Cords
- > Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report
- > Full 180 Day Parts and Labor Warranty
Below is a List of Diagnostic Tests Performed On This System
- Filter Test: The test moves the filter into the light path and measures the relative absorbance in the filter position.
- Dark Current Test: The test measures the dark current from the detector optic.
- Holmium Oxide Test: The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
- Lamp Intensity Test: The test verifies functionality of the UV lamp at various wavelengths.
- Wavelength Intensity Test: The test confirms the accuracy of wavelengths measured.
- Baseline Noise Test: The test measures the amount of baseline noise drift identified by the detector.
- Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
- Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.
- Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.
- Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
- 4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the Agilent 1260 Infinity II LC System w/ VWD & RID.
Agilent 1260 Infinity II LC System w/ VWD & RID Specifications
VWD Detector
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Specification
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Detection Type
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Double-beam photometer
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Light Source
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Deuterium lamp
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Wavelength Range
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190 - 600 nm
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Noise
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< ± 0.25 x 10^-5 AU, at 230 nm (single wavelength detection)
< ± 0.80 x 10^-5 AU, at 230 nm and 254 nm (dual wavelength detection)
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Drift
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< 1 x 10^-4 AU/h at 230 nm
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Linearity
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> 2.5 AU upper limit
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Wavelength Accuracy
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± 1 nm, self-calibration with deuterium lines, verification with holmium oxide filter
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Wavelength Precision
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< ± 0.1 nm
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Slit Width
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6.5nm typical over whole wavelength range
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Time Programmable
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Wavelength, polarity, peak width, lamp on/off
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Flow Cell
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Standard: 14 µl volume, 10 mm cell path length and 40 bar (588 psi) pressure maximum
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Spectral Tools
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Stop-flow wavelength scan
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RID Detector
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Specification
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Detection Type
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Refractive Index
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Refractive Index Range
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1.00 - 1.75 RIU, calibrated
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Measurement Range
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± 600 x 10^-6 RIU
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Optics Temperature Control
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5°C above ambient to 55°C
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Sample Cell
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Volume: 8µl
Maximum pressure: 5 bar (0.5 MPa)
Maximum flow rate: 5 ml/min
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Valves
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Automatic purge and automatic solvent cycle
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Volumes
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Inlet port to sample cell 62 µl
Inlet port to outlet port 590 µl
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Liquid Contact Materials
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316 stainless steel, PTFE and quartz glass
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pH Range
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2.3 - 9.5
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Short Term Noise
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< ± 1.25 x 10^-9 RIU
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Drift
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< 200 x 10^-9 RIU/hr
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Time Programmable
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Polarity and peak width
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Maximum Data Rate
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74 Hz
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Click Here to View More Specifications for the Agilent 1260 Infinity II LC System w/ VWD
Quaternary Pump
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Specification
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Hydraulic System
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Dual piston in series pump with servo-controlled variable stroke driven, powe transmission by gears and ball screws, floating pistons
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Settable Flow Range
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Set points 0.001 - 10 ml/min, in 0.001 ml/min increments
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Recommended Flow Range
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0.02 - 10.0 ml/min
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Flow Precision
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≤ 0.07% RSD or ≤ 0.02 min SD
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Flow Accuracy
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± 1% or 10 µl/min, whichever is greater, pumping degassed H2O at 10 MPa (100 bar)
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Pressure Operating Range
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Up to 60 MPa (600 bar, 8700 psi) up to 5 ml/min
Up to 20 MPa (200 bar, 2950 psi) up to 10 ml/min
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Pressure Pulsation
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< 2% amplitude (typically < 1.0%) or < 0.3 MPa (3 bar, 44 psi), whichever is greater
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Compressibility Compensation
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User-selectable, based on mobile phase compressibility
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Recommended pH Range
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1.0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
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Gradient Formation
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Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve
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Delay Volume
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600 - 900 µl, dependent on back pressure; measured with water at 1 ml/min (water/caffeine tracer)
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Settable Composition Range
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0 - 100% in 0.1% increments
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Composition Precision
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< 0.2% RSD or < 0.04 min SD, whatever is greater
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Integrated Degassing Unit
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Number of channels: 4
Internal volume per channel: 1.5 ml
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Multisampler
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Specification
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Injection Range
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0.1 - 90 µl in 0.1 µl increments
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Injection Precision
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< 0.15% RSD or SD < 10nL, whatever is greater
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Injection Linearity
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0.9999 in the range of 0.1 - 100 µl
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Pressure Range
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Up to 800 bar
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Sample Viscosity Range
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0.2 - 5 cp
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Sample Capacity
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1H Drawer: up to 8 drawers and 16 positions
Shallow well plates (MTP)
2H Drawer: up to 4 drawers and 8 positions MTP, deep well plates, vials, Eppendorf
3H Drawer: up to 2 drawers and 4 positions MTP, deep well plates, vials up to 6 ml, Eppendorf
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Injection Cycle Time
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< 10 seconds using following conditions:
Default draw speed: 100 ul/min Default eject speed: 400 ul/min Injection volume: 1 ul
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Multiwash
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Outer needle wash and seat backflush for carryover reduction with up to 3 different solvents
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Carry Over
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< 0.003% (30 ppm) Multisampler Standard Needle
<0.0009% (3 ppm) Multisampler Multiwash
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Multicolumn Thermostat
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Specification
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Temperature Range
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Ambient -10°C to 85°C (minimum 4°C)
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Temperature Stability
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± 0.1°C
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Temperature Accuracy
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± 0.5°C (with calibration for 40°C)
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Temperature Precision
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0.05°C
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Independent Temperature Zones
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2 in single device
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Column Capacity
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4 columns of up to 300 mm length plus InfinityLab Quick-Connect fittings or pre-column The number of precolumn Quick-Connect Heat Exchangers is scalable - each column can be equipped with individual heat exchanger for best performance 4-column selector valve is available to access each column without replumbing
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Heat-Up / Cool-Down Time
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5 min from ambient to 40°C
10 min from 40°C to 20°C < 25 min from 25°C to 85°C
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Computer Specifications
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Processor
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Intel Core I5-8500 CPU
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RAM
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32.0 GB
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Hard Drive
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500GB Internal SSD
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Operating System
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Windows 11 Professional, 64-bit
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- Verify uniformity of main and residential firmware across all components, updating as necessary to ensure compatibility
- Confirm successful communication between software and the HPLC system, validating data transfer integrity
- Check the degasser’s internal vacuum to confirm it reaches the specified vacuum level for optimal performance
- Replace inlet, outlet, purge valve, and pump seals in the system pump to maintain reliable operation
- Ensure system pump passes pressure and leak tests, confirming secure and efficient performance
- Replace all capillary tubing with new OEM stainless steel tubing for durability and precision
- Remove autosampler transport assembly, replace X and Y bearings, and thoroughly clean carrier rails to remove debris and contaminants
- Replace metering valve seal, autosampler needle, and needle seat for consistent sampling accuracy
- Test column compartment switching valve functionality and replace its seal if necessary
- Confirm column compartment passes thermostat test to maintain precise temperature control
- Remove and clean internal detector optics for clear and accurate signal detection
- Replace UV and Vis detector lamps to ensure optimal light source stability and performance
- Verify flow cell absorbance meets manufacturer’s specifications for accurate measurement
- Confirm system response to isocratic test sample, ensuring stable and reproducible results
Technician Inspection and Recertification Checklist: HPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
Task
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Completed?
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Remarks?
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Verify firmware uniformity and update as necessary
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Completed
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Confirm software communication with HPLC system
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Completed
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Check degasser vacuum level for optimal performance
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Completed
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Replace inlet, outlet, purge valve, and pump seals
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Completed
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Ensure pump passes pressure and leak tests
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Completed
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Replace all capillary tubing with OEM stainless steel tubing
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Completed
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Remove autosampler transport assembly, replace bearings, and clean rails
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Completed
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Replace metering valve seal, autosampler needle, and needle seat
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Completed
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Test column compartment switching valve and replace seal if needed
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Completed
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Confirm thermostat test in column compartment
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Completed
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Clean internal detector optics
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Completed
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Replace UV and Vis detector lamps
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Completed
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Verify flow cell absorbance meets manufacturer’s specifications
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Completed
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Confirm system response to isocratic test sample
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Completed
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To View More of Our Recertification Protocol Click Here
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