 |
 |
 |
|
Agilent 1260 Infinity II LC System
The Agilent 1260 Infinity II LC System provides a liquid chromatograph offering ultra fast and high resolution separation capability for consistently accurate results. The 1260 Infinity II LC System delivers several ease-of-use features, making it a standard system for routine analysis. With an operating pressure of up to 600 bar, the 1260 Infinity II Binary Pump is suitable for multiple LC applications, handling columns over the flow rate range of up to 5 ml/min. The 1260 Infinity II Multisampler can handle both vials and microtiter plates with ease and efficiency, and is designed for low carryover, as you can take clean to a whole new level with the multi-wash capability, cleaning all relevant injection parts between runs. This integrated feature flushes the injection needle outside with three solvents, and uses seat back flush procedures to reduce carryover to less than 9 ppm.
The 1260 Infinity II Diode-Array Detector WR features a 120 Hz data acquisition rate, perfectly suited for fast LC applications. This DAD WR detector is equipped with long-life deuterium lamps, which allow for the highest sensitivity and lowest detection limits over a wavelength range of 190-950 nm. The Agilent 1260 Infinity II LC System also allows for superior temperature control, with a range of 4°C (minimum of 20°C below ambient) to 110°C on certain columns, allowing for selectivity flexibility and reduces solvent viscosity to allow even faster separation. The 1260 Infinity II LC System is also equipped with the G1770A Universal Valve Drive, which enable users to adjust the system based on their application's needs with the simple change of an Agilent Quick Change Valve Head. Included is the G4235A Bioinert 12 Position / 13 Port Solvent Selection Valve Head for the selection or fractionation of solvents.
The Agilent 1260 Infinity II LC System can be fully controlled from the Agilent MassHunter Workstation Software, which allows for user-friendly operation in data acquisition, analysis, instrument control, automation, and more.
This Agilent system is in excellent condition and will be fully tested to perform at factory
specifications before being shipped.
The Agilent 1260 Infinity II LC System includes:
- > G7112B Binary Pump
- > G7115A DAD WR Detector
- > G7167A Multisampler
- > G7116B Multicolumn Thermostat
- > G1170A Universal Valve Drive
- > G4235A Bioinert 12 Position / 13 Port Solvent Selection Valve Head
- > Datastation w/ MassHunter Workstation Software
- > Can/Crossover Cables
- > Agilent Lan Interface Card Built-In
- > Solvent Tubing
- > 4 x Bottles, Caps and Solvent Filters
- > New Stainless Steel Capillaries
- > 1/4" Wrench & Mobile Phase Priming Syringe
- > Waste Tubing
- > All Power Cords
- > Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report
- > Full 180 Day Parts and Labor Warranty
Below is a List of Diagnostic Tests Performed On This System
- Filter Test: The test moves the filter into the light path and measures the relative absorbance in the filter position.
- Dark Current Test: The test measures the dark current from the detector optic.
- Holmium Oxide Test: The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
- Lamp Intensity Test: The test verifies functionality of the UV lamp at various wavelengths.
- Wavelength Intensity Test: The test confirms the accuracy of wavelengths measured.
- Baseline Noise Test: The test measures the amount of baseline noise drift identified by the detector.
- Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
- Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.
- Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.
- Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
- 4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the 1260 Infinity II LC System.
Agilent 1260 Infinity II LC System Specifications
| DAD WR Detector |
|
Detection Type
|
1024-element photodiode array |
| Light Source |
Deuterium and tungsten lamps |
| Wavelength Range |
190 - 950 nm |
| Short Term Noise (ASTM) Single and Multi-Wavelength |
< ± 0.7 x 10^-5 AU at 254 and 750 nm |
| Drift |
< 0.9 x 10^-3 AU/h at 254 nm |
| Linear Absorbance Range |
> 2 AU (5%) at 265 nm |
| Wavelength Accuracy |
± 1 nm |
| Slit Width |
1, 2, 4, 8, 16 nm |
| Diode Width |
< 1 nm |
| Binary Pump |
| Settable Flow Range |
Set points 0.001 - 5 ml/min, in 0.001 ml/min increments |
| Recommended Flow Range |
0.05 - 5.0 ml/min
|
| Flow Precision |
≤ 0.07% RSD or ≤ 0.02 min SD |
| Flow Accuracy |
± 1% or 10 µl/min, whichever is greater |
| Pressure Operating Range |
Up to 60 MPa (600 bar, 8702 psi) up to 5 ml/min |
| Pressure Pulsation |
< 2% amplitude (typically < 1.3%) or < 0.3 MPa (3 bar, 44 psi), whichever is greater |
| Composition Range |
Settable range: 0 - 100%
Recommended range: 1 - 99% or 5 µl/min per channel |
| Composition Precision |
< 0.15 % RSD or < 0.04 min SD |
| Composition Accuracy |
± 0.35% absolute, at 2 ml/min, at 10 MPa (100 bar) |
| Multisampler |
| Injection Range |
0.1 - 90 µl in 0.1 µl increments |
| Injection Precision |
< 0.15% RSD or SD < 10nL, whatever is greater |
| Pressure Range |
Up to 800 bar |
| Sample Viscosity Range |
0.2 - 5 cp |
| Sample Capacity |
1H Drawer: up to 8 drawers and 16 positions
Shallow well plates (MTP)
2H Drawer: up to 4 drawers and 8 positions
MTP, deep well plates, vials, Eppendorf
3H Drawer: up to 2 drawers and 4 positions
MTP, deep well plates, vials up to 6 ml, Eppendorf |
| Injection Cycle Time |
< 10 seconds using following conditions:
Default draw speed: 100 ul/min
Default eject speed: 400 ul/min
Injection volume: 1 ul |
| Multiwash |
Outer needle wash and seat backflush for carryover reduction with up to 3 different solvents |
| Carry Over |
< 0.003% (30 ppm) Multisampler Standard Needle
<0.0009% (3 ppm) Multisampler Multiwash |
| Multicolumn Thermostat |
| Temperature Range |
4°C to 110°C (minimum 20°C below ambient) |
| Temperature Stability |
± 0.03°C |
| Temperature Accuracy |
± 0.5°C (with calibration) |
| Temperature Precision |
0.05°C |
| Heat-Up / Cool-Down Time |
5 min from ambient to 40°C
10 min from 40°C to 20°C
< 30 min from 25°C to 100°C |
| Solvent Selection Valve |
| Maximum Pressure |
210 bar |
| Fittings |
10-32 |
| Port-Port Volume |
16.4 ul |
| Liquid Contact |
PEEK, Ceramic |
| pH Range |
0-14 |
- Verify uniformity of main and residential firmware across all components, updating as necessary to ensure compatibility
- Confirm successful communication between software and the HPLC system, validating data transfer integrity
- Check the degasser’s internal vacuum to confirm it reaches the specified vacuum level for optimal performance
- Replace inlet, outlet, purge valve, and pump seals in the system pump to maintain reliable operation
- Ensure system pump passes pressure and leak tests, confirming secure and efficient performance
- Replace all capillary tubing with new OEM stainless steel tubing for durability and precision
- Remove autosampler transport assembly, replace X and Y bearings, and thoroughly clean carrier rails to remove debris and contaminants
- Replace metering valve seal, autosampler needle, and needle seat for consistent sampling accuracy
- Test column compartment switching valve functionality and replace its seal if necessary
- Confirm column compartment passes thermostat test to maintain precise temperature control
- Remove and clean internal detector optics for clear and accurate signal detection
- Replace UV and Vis detector lamps to ensure optimal light source stability and performance
- Verify flow cell absorbance meets manufacturer’s specifications for accurate measurement
- Confirm system response to isocratic test sample, ensuring stable and reproducible results
Technician Inspection and Recertification Checklist: HPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
|
Task
|
Completed?
|
Remarks?
|
|
Verify firmware uniformity and update as necessary
|
Completed
|
|
|
Confirm software communication with HPLC system
|
Completed
|
|
|
Check degasser vacuum level for optimal performance
|
Completed
|
|
|
Replace inlet, outlet, purge valve, and pump seals
|
Completed
|
|
|
Ensure pump passes pressure and leak tests
|
Completed
|
|
|
Replace all capillary tubing with OEM stainless steel tubing
|
Completed
|
|
|
Remove autosampler transport assembly, replace bearings, and clean rails
|
Completed
|
|
|
Replace metering valve seal, autosampler needle, and needle seat
|
Completed
|
|
|
Test column compartment switching valve and replace seal if needed
|
Completed
|
|
|
Confirm thermostat test in column compartment
|
Completed
|
|
|
Clean internal detector optics
|
Completed
|
|
|
Replace UV and Vis detector lamps
|
Completed
|
|
|
Verify flow cell absorbance meets manufacturer’s specifications
|
Completed
|
|
|
Confirm system response to isocratic test sample
|
Completed
|
|
To View More of Our Recertification Protocol Click Here
|
|
|
 |
|
 |
|
