Agilent 1260 Infinity I HPLC System w/ DAD & FLD

Standard: 13 µL volume, 10 mm cell path length and 120 bar (1740 psi) pressure maximum

FLD Detector

Specification

Detection Type

Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis

Performance Specifications

Single wavelength operation: • RAMAN (H2O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell • RAMAN (H2O) > 3000 (noise reference measured at dark value) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell Dual wavelength operation: RAMAN (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell.

Light source

Xenon Flash Lamp, normal mode 20 W, economy mode 5 W, lifetime 4000 h

Pulse frequency

296 Hz for single signal mode 74 Hz for economy mode

Excitation Monochromator

Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 300 nm

Emission Monochromator

Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 400 nm

Reference System

In-line excitation measurement

Timetable programing

up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters

Spectrum acquisition

Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200 – 400 nm, 10 nm step) Step size: 1 – 20 nm Spectra storage: All

Wavelength characteristic

Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting

Flow cells

Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz