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Agilent 1260 Infinity I HPLC System w/ DAD & FLD
The Agilent 1260 Infinity I HPLC System w/ DAD & FLD offers
the most flexibility for solvent selection and automation in HPLC method development, research and all HPLC applications requiring continuous access to a wide range of solvent choices. This system is ideally suited for multi-method, high-throughput workflows and offers convenient access to two solvents for isocratic or gradient analysis, for rapid method development and speeding up preparation of mobile phases and flushing the HPLC system.
The Agilent 1260 Infinity HPLC System's binary pump is comprises two identical pumps integrated into one housing. Binary gradients are created by high-pressure mixing. Typical applications are high throughput methods with fast gradients on high resolution 2.1 mm columns. The pump is capable of delivering flow in the range of 0.1 - 5 mL/min against up to 600 bar. Degassing of the solvents is done with the High Performance Degasser, which comprises four separate vacuum chambers with semipermeable tubings, a vacuum pump, and control assembly. The 1260 Infinity Autosampler is designed to offer the well-established flow-through design with variable volume injection and to achieve extremely low carryover, providing increased analysis speed with sensitivity, resolution, and precision. Paired with the autosampler is a thermostatted column compartment and ALS thermostat to serve as a temperature management system providing heating and cooling in order to meet extreme requirements of retention time reproducibility.
The featured Diode-Array Detector (DAD) is designed for the highest optical performance, GLP compliance, and easy maintenance. The DAD provides greatly enhanced sensitivity and baseline stability using a cartridge cell system with optofluidic waveguides. This detector provides for a wavelength range of 190 - 950 nm, a data acquisition rate of up to 80 Hz, and a programmable 1, 2, 4, 8, or 16 nm slit for rapid optimization of sensitivity, linearity, and spectral resolution. Also featured is the Fluorescence Detector (FLD), which brings high-sensitivity and selection through simultaneous multi-wavelength detection. This easy-to-use detector provides quantitative data and fluorescence spectra from a single run. Some of the features of the FLD Detector include; a flash lamp for highest intensity and lowest detection limit, multi-wavelength mode for on-line spectra, spectra acquisition and simultaneous multi-signal detection, easy front access to the flow cell for fast replacement, and built-in wavelength accuracy verification. Users may also use an optional cuvette for off-line measurements.
The Agilent 1260 Infinity I HPLC System w/ DAD & FLD can be fully controlled from the included Agilent ChemStation Workstation Software, which allows for user-friendly operation in data acquisition, analysis, instrument control, automation, and more.
This Agilent system is in excellent condition and will be fully tested to perform at factory
specifications before being shipped.
The Agilent 1260 Infinity I HPLC System w/ DAD & FLD includes:
- > G1312B Binary Pump
- > G1329B Autosampler
- > G1330B ALS Thermostat
- > G1316C TCC
- > G4225A HiP Degasser
- > G4212B DAD Detector
- > G1321B FLD Detector
- > Windows 11 Professional 64-Bit Data Station with Flatscreen Monitor
- > ChemStation Version 4.3 Software Installed
- > Can/Crossover Cables
- > Agilent Lan Interface Card Built-In
- > Solvent Tubing
- > 4 x Bottles, Caps and Solvent Filters
- > New Stainless Steel Capillaries
- > 1/4" Wrench & Mobile Phase Priming Syringe
- > Waste Tubing
- > All Power Cords
- > Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report
- > Full 180 Day Parts and Labor Warranty
Below is a List of Diagnostic Tests Performed On This System
- Filter Test: The test moves the filter into the light path and measures the relative absorbance in the filter position.
- Dark Current Test: The test measures the dark current from the detector optic.
- Holmium Oxide Test: The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
- Lamp Intensity Test: The test verifies functionality of the UV lamp at various wavelengths.
- Wavelength Intensity Test: The test confirms the accuracy of wavelengths measured.
- Baseline Noise Test: The test measures the amount of baseline noise drift identified by the detector.
- Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
- Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.
- Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.
- Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
- 4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the 1260 Infinity HPLC System.
Agilent 1260 Infinity I HPLC System w/ DAD & FLD Specifications
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DAD Detector
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Specification
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Detection Type
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1024-element photodiode array
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Light Source
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Deuterium and tungsten lamps
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Wavelength Range
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190 - 640 nm
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Short Term Noise (ASTM) Single and Multi-Wavelength
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< ± 0.7 × 10^-5 AU at 254 nm and 750 nm
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Drift
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< 0.9 × 10^-3 AU/hr at 254 nm
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Linear Absorbance Range
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> 2.0 AU (5%) at 265 nm
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Wavelength Accuracy
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± 1 nm
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Wavelength Bunching
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1 - 400 nm
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Slit Width
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1, 2, 4, 8, 16 nm / programmable slit
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Diode Width
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< 1 nm
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Signal Data Rate
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Up to 80 Hz
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Spectra Data Rate
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Up to 80 Hz
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Flow Cell
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Standard: 13 µL volume, 10 mm cell path length and 120 bar (1740 psi) pressure maximum
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Control and Data Evaluation
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Agilent ChemStation for LC
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FLD Detector
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Specification
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Detection Type
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Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
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Performance Specifications
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Single wavelength operation: • RAMAN (H2O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell • RAMAN (H2O) > 3000 (noise reference measured at dark value) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell Dual wavelength operation: RAMAN (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell.
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Light source
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Xenon Flash Lamp, normal mode 20 W, economy mode 5 W, lifetime 4000 h
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Pulse frequency
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296 Hz for single signal mode 74 Hz for economy mode
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Excitation Monochromator
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Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 300 nm
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Emission Monochromator
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Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 400 nm
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Reference System
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In-line excitation measurement
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Timetable programing
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up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters
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Spectrum acquisition
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Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200 – 400 nm, 10 nm step) Step size: 1 – 20 nm Spectra storage: All
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Wavelength characteristic
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Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting
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Flow cells
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Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz
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Click Here to View More Specifications for the Agilent 1260 Infinity HPLC System
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Binary Pump
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Specification
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Hydraulic System
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Two dual piston in series pumps with servo-controlled variable stroke drive, power transmission by gears and ball screws, floating pistons
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Setable Flow Range
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0.001 - 5 mL/min, in 0.001 mL/min increments
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Flow Range
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0.05 - 5.0 mL/min
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Flow Precision
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< 0.07% RSD or < 0.02 min SD, whatever is greater
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Flow Accuracy
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± 1% or 10 µl/min, what ever is greater
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Pressure Operating Range
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0 - 60 MPa (0 - 600 bar, 0 - 8,700 psi) up to 5 mL/min
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Pressure Pulsation
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< 2% amplitude or < 3 bar at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar, 147 psi)
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Compressibility Compensation
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Pre-defined, based on mobile phase compressibility
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Recommended pH Range
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1.0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
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Gradient Formation
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High-pressure binary mixing
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Delay Volume
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Standard delay volume configuration: 600 - 800 µl, (includes 400 µl mixer), dependent on back pressure
Low delay volume configuration: 120 µl
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Autosampler
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Specification
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Injection Range
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0.1 - 100 µl in 0.1 µl increments
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Precision
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< 0.25% from 5 - 40µl
< 0.5% from 2 - 5µl
< 0.7% from 1 - 2µl
< 1.5% from 0.5 - 1µl
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Pressure Range
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Up to 600 bar (8,700 psi)
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Sample Viscosity Range
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0.2 - 5 cp
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Sample Capacity
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2 x well plates (MTP) + 10 x 2 ml vials
108 x 2 ml vials in 2 x 54 vial plate plus 10 additional 2 ml vials
30 x 6 ml vials in 2 x 15 vial plate
100 Micro vial tray plus 10 additional 2 ml vials
54 Eppendorf tubes (0.5/1.5/2 ml) in 2 x 27 Eppendorf tube plates
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Injection Cycle Time
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Typically < 21 seconds using default conditions and injection volume of 5µl
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Carryover
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Typically < 0.004%
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ALS Thermostat
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Specification
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Temperature Range
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4°C to 40°C in 1°C increments
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Temperature Accuracy at Ambient Temperatures < 25°C and Humidity < 50%
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3°C to 8°C at a setpoint of 4°C
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Temperature Accuracy at Ambient Temperatures > 30°C and Humidity > 60%
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3°C to 9°C at a setpoint of 4°C
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Column Compartment
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Specification
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Temperature Range
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Ambient -10°C to 80°C at flow rates up to 5 mL/min
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Temperature Stability
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± 0.15°C
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Temperature Accuracy
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± 0.8°C
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Warm-Up/Cool-Down Time
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5 minutes from ambient to 40°C
10 minutes from 40°C to 20°C
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High Performance Degasser
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Specification
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Number of Solvent Channels
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4
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Flow Range
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0 - 10 mL/min per channel
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Internal Volume Per Channel
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0.45 mL per channel
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Materials in Contact with Solvent
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TFE/PDD Copolymer, FEP, PEEK
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pH Range
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1 - 14
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Computer Specifications
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Processor
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Intel Core I5-8500 CPU
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RAM
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32.0 GB
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Hard Drive
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500GB Internal SSD
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Operating System
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Windows 11 Professional, 64-bit
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- Verify uniformity of main and residential firmware across all components, updating as necessary to ensure compatibility
- Confirm successful communication between software and the HPLC system, validating data transfer integrity
- Check the degasser’s internal vacuum to confirm it reaches the specified vacuum level for optimal performance
- Replace inlet, outlet, purge valve, and pump seals in the system pump to maintain reliable operation
- Ensure system pump passes pressure and leak tests, confirming secure and efficient performance
- Replace all capillary tubing with new OEM stainless steel tubing for durability and precision
- Remove autosampler transport assembly, replace X and Y bearings, and thoroughly clean carrier rails to remove debris and contaminants
- Replace metering valve seal, autosampler needle, and needle seat for consistent sampling accuracy
- Test column compartment switching valve functionality and replace its seal if necessary
- Confirm column compartment passes thermostat test to maintain precise temperature control
- Remove and clean internal detector optics for clear and accurate signal detection
- Replace UV and Vis detector lamps to ensure optimal light source stability and performance
- Verify flow cell absorbance meets manufacturer’s specifications for accurate measurement
- Confirm system response to isocratic test sample, ensuring stable and reproducible results
Technician Inspection and Recertification Checklist: HPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
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Task
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Completed?
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Remarks?
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Verify firmware uniformity and update as necessary
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Completed
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Confirm software communication with HPLC system
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Completed
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Check degasser vacuum level for optimal performance
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Completed
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Replace inlet, outlet, purge valve, and pump seals
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Completed
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Ensure pump passes pressure and leak tests
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Completed
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Replace all capillary tubing with OEM stainless steel tubing
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Completed
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Remove autosampler transport assembly, replace bearings, and clean rails
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Completed
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Replace metering valve seal, autosampler needle, and needle seat
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Completed
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Test column compartment switching valve and replace seal if needed
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Completed
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Confirm thermostat test in column compartment
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Completed
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Clean internal detector optics
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Completed
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Replace UV and Vis detector lamps
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Completed
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Verify flow cell absorbance meets manufacturer’s specifications
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Completed
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Confirm system response to isocratic test sample
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Completed
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To View More of Our Recertification Protocol Click Here
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