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Agilent 1200 HPLC System with Diode Array Detector and Fluorescence Detector
The Agilent 1200 HPLC System guarantees the most efficient
mixing and pulse-free solvent delivery for consistently fast and accurate
results. The system’s optimized design using a quaternary pump allows variable
stroke volumes for stable solvent flow. This electronically activated inlet
valve runs synchronous to the pistons to eliminate vapor formation in volatile
solvent, making the Agilent 1200 both remarkably easy-to-use and
maintenance-free. It also features a high-speed proportioning valve and a
pre-configured degasser, which allow convenient low-pressure mixing and a large
solvent choice flexibility. The HPLC System’s vacuum degasser ensures reduced
operating costs without the use of helium and optimum instrument performance.
The Agilent 1200 Diode Array Detector verifies separation
quality with peak purity, multiple signals, and spectral libraries during your
analytical quantification, offering an extended wavelength range with the
highest sensitivity. The VWD and diode array detectors allow for automated
wavelength verification for performance tracking. The Agilent 1200’s
temperature management system ensures optimum baseline stability-even under
rough ambient conditions. This detector features superb illumination using
combined deuterium and tungsten lamps for the highest intensity and lowest
detection limit from 190 to 950 nm, 1024 diodes and 1-nm slit for highest
spectral resolution. The programmable slit helps you explore the spectral
landscape for faster sample characterization, ensuring higher sensitivity or
lower baseline noise as necessary on the 1200. Slit settings can be stored and
reported from 1 – 16 nm with raw data for GLP traceability.
The unit can be operated with a hand-held controller or
computer running Clarity software. The HPLC System features extensive
diagnostics with compatible data analysis software for on-site and remote
trouble-shooting.
This Agilent system is in excellent condition and will be fully tested to perform at factory
specifications before being shipped.
Recertified Agilent 1200 System
The Agilent 1200 HPLC System w/ DAD & FLD includes:
- > G1311A Quaternary Pump or G1312A Binary Pump
- > G1329A Thermal Autosampler
- > G1330B Thermal Unit
- > G1315C DAD Detector
- > G1321A FLD Detector
- > G1316A TCC
- > G1322A Degasser
- > Windows 11 Professional 64-Bit Data Station with Flatscreen Monitor
- > Chemstation version 4.3 Software installed
- > Can/Crossover Cables
- > Agilent G1369 Lan Interface Card
- > Solvent Tubing
- > 4 x Bottles, Caps and Solvent Filters
- > New Stainless Steel Capillaries
- > 1/4" Wrench & Mobile Phase Priming Syringe
- > Waste Tubing
- > All Power Cords
- > Agilent 4 Peak Test w/ Lab Advisor Diagnostics Report
- > Full 180 Day Parts and Labor Warranty
Below is a List of Diagnostic Tests Performed On This System
- Filter Test: The test moves the filter into the light path and measures the relative absorbance in the filter position.
- Dark Current Test: The test measures the dark current from the detector optic.
- Holmium Oxide Test: The test measures the Holmium spectrum from the built-in Holmium filter. The spectrum is evaluated for peaks at different wavelengths.
- Lamp Intensity Test: The test verifies functionality of the UV lamp at various wavelengths.
- Wavelength Intensity Test: The test confirms the accuracy of wavelengths measured.
- Baseline Noise Test: The test measures the amount of baseline noise drift identified by the detector.
- Pressure Test: The pressure test is a quick, built-in test designed to demonstrate the pressure tightness (up to 400 bar) of the system.
- Leak Test: The leak test is a built-in troubleshooting designed to demonstrate the leak-tightness of the pump.
- Thermostat Test: Evaluates the cooling and heating performance of the two Peltier elements.
- Injector Steps: Performs each movement of the sampling sequence under manual control. This is useful for confirming autosampler functionality.
- 4 Peak Test (Isocratic Standard): Standard test published by Agilent to confirm complete functionality of the 1200 HPLC System.
Agilent 1200 HPLC System with DAD Detector Specifications
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Type
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Specification
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Detection type
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1024-element photodiode array
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Light Source
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Deuterium and tungsten lamps
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Wavelength Range
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190 - 950 nm
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Short term noise (ASTM) Single
and Multi-Wavelength
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± 1 × 10-5AU at 254 and 750 nm
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Drift
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2 × 10-3AU/hr at 254 nm
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Linear Absorbance range
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> 2 AU (upper limit)
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Wavelength accuracy
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± 1 nm
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Slit width
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1, 2, 4 , 8, 16 nm
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Diode Width
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< 1 nm
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Flow Cell
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Standard: 13 µl volume, 10 mm cell
path length and 120 bar (1760 psi)
pressure maximum
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Control and Data Evaluation
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Agilent ChemStation for LC
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Analog Outputs
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Recorder/integrator: 100 mV or 1 V,
output range 0.001 – 2 AU, two
outputs
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Communications
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Controller-area network (CAN), GPIB,
RS-232C, APG Remote: ready, start,
stop and shut-down signals, LAN
optional
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Safety and Maintenance
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Extensive diagnostics, error detection
and display (through control module
and ChemStation), leak detection, safe
leak handling, leak output signal for
shutdown of pumping system. Low
voltages in major maintenance areas.
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GLP Features
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Early maintenance feedback (EMF) for
continuous tracking of instrument
usage in terms of lamp burn time with
user-settable limits and feedback
messages. Electronic records of
maintenance and errors. Verification of
wavelength accuracy with built-in
holmium oxide filter.
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Housing
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All Materials recyclable
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Agilent 1200 FLD Detector Specifications
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Detection Type
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Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
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Performance Specifications
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10 fg Anthracene, Ex=250 nm, Em=400 nm* RAMAN single wavelength (H2O) > 500 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime) RAMAN dual wavelength (H2O) > 300 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime)
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Light source
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Xenon Flash Lamp, normal mode 20 W, economy mode 5 W
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Pulse frequency
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296 Hz for single signal mode 74 Hz for spectral mode
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Excitation Monochromator
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Range: 200 nm - 700 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 300 nm
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Emission Monochromator
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Range: 280 nm - 900 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 400 nm
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Reference System:
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in-line excitation measurement
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Timetable programing
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up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters
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Spectrum acquisition:
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Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200-400 nm, 10 nm step) Step size: 1-20 nm Spectra storage: All
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Wavelength characteristic
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Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting
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Flow cells
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Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz
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Control and data evaluation
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Agilent ChemStation for LC, Agilent Instant Pilot G4208A or Agilent Control Module G1323B with limited spectral data analysis and printing of spectra
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Communications
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Controller-area network (CAN), GPIB, RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals
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Detection Type
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Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
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Performance Specifications
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10 fg Anthracene, Ex=250 nm, Em=400 nm* RAMAN single wavelength (H2O) > 500 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime) RAMAN dual wavelength (H2O) > 300 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime)
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Light source
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Xenon Flash Lamp, normal mode 20 W, economy mode 5 W
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Pulse frequency
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296 Hz for single signal mode 74 Hz for spectral mode
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Computer Specifications
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Processor
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Intel Core I5-8500 CPU
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RAM
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32.0 GB
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Hard Drive
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500GB Internal SSD
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Operating System
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Windows 11 Professional, 64-bit
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- Verify uniformity of main and residential firmware across all components, updating as necessary to ensure compatibility
- Confirm successful communication between software and the HPLC system, validating data transfer integrity
- Check the degasser’s internal vacuum to confirm it reaches the specified vacuum level for optimal performance
- Replace inlet, outlet, purge valve, and pump seals in the system pump to maintain reliable operation
- Ensure system pump passes pressure and leak tests, confirming secure and efficient performance
- Replace all capillary tubing with new OEM stainless steel tubing for durability and precision
- Remove autosampler transport assembly, replace X and Y bearings, and thoroughly clean carrier rails to remove debris and contaminants
- Replace metering valve seal, autosampler needle, and needle seat for consistent sampling accuracy
- Test column compartment switching valve functionality and replace its seal if necessary
- Confirm column compartment passes thermostat test to maintain precise temperature control
- Remove and clean internal detector optics for clear and accurate signal detection
- Replace UV and Vis detector lamps to ensure optimal light source stability and performance
- Verify flow cell absorbance meets manufacturer’s specifications for accurate measurement
- Confirm system response to isocratic test sample, ensuring stable and reproducible results
Technician Inspection and Recertification Checklist: HPLC Systems
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
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Task
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Completed?
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Remarks?
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Verify firmware uniformity and update as necessary
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Completed
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Confirm software communication with HPLC system
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Completed
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Check degasser vacuum level for optimal performance
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Completed
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Replace inlet, outlet, purge valve, and pump seals
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Completed
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Ensure pump passes pressure and leak tests
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Completed
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Replace all capillary tubing with OEM stainless steel tubing
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Completed
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Remove autosampler transport assembly, replace bearings, and clean rails
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Completed
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Replace metering valve seal, autosampler needle, and needle seat
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Completed
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Test column compartment switching valve and replace seal if needed
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Completed
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Confirm thermostat test in column compartment
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Completed
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Clean internal detector optics
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Completed
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Replace UV and Vis detector lamps
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Completed
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Verify flow cell absorbance meets manufacturer’s specifications
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Completed
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Confirm system response to isocratic test sample
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Completed
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To View More of Our Recertification Protocol Click Here
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