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Agilent 1200 HPLC G1321A Fluorescence Detector
Wavelength Range (Emission)
280 to 900 nm
Wavelength Range (Excitation)
200 to 700 nm
Light Source
Xenon Flash Lamp
- Designed for highest optical performance, GLP compliance, and easy maintenance
- Features a high-intensity flash lamp to ensure the lowest detection limits
- Multi-wavelength mode enables on-line spectral acquisition and simultaneous multi-signal detection
- Optional cuvette available for off-line measurements
- Easy front access to the flow cell allows for quick replacement
- Built-in wavelength accuracy verification to maintain reliable results
- Compatible with Agilent ChemStation for LC and other control modules for enhanced data evaluation and control
The Agilent 1200 HPLC G1321A FLD Detector is engineered for superior optical performance, GLP compliance, and streamlined maintenance. This multi-signal fluorescence detector features rapid online scanning and detailed spectral data analysis capabilities. Key highlights include a high-intensity flash lamp to achieve the lowest detection limits and a multi-wavelength mode that allows for on-the-fly spectral acquisition and simultaneous signal detection. Users can benefit from the optional cuvette for off-line measurements, easy access to the flow cell for quick replacements, and built-in wavelength accuracy verification tools.
Performance is robust, with specifications including a detection limit of 10 fg for Anthracene with excitation at 250 nm and emission at 400 nm. The system operates using a Xenon Flash Lamp with a normal output of 20 W and an economy mode of 5 W, with pulse frequencies adjusted according to the mode selected. Both excitation and emission monochromators cover a wide wavelength range and are equipped with fixed 20 nm bandwidth concave holographic gratings.
The detector supports up to four signal wavelengths, adjustable PMT gain, and flexible baseline behaviors in its timetable programming features. For data management, it utilizes Agilent’s ChemStation software or can be controlled via the Agilent Instant Pilot G4208A or Control Module G1323B, offering limited spectral data analysis and spectrum printing capabilities. Connection options include CAN, GPIB, RS-232C, LAN, and APG Remote for easy integration into existing systems.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The Agilent 1200 HPLC G1321A Fluorescence Detector includes:
- > Firmware Version 07.02 (Can be changed upon request)
- > Calibration Report
- > 8μl Flow Cell
- > Lab Advisor Calibration Report
- > Lan Cable / Power Cord
- > Operator's Manual
- > Full 180 Day Parts and Labor Warranty
Agilent 1200 HPLC G1321A Fluorescence Detector Specifications
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Detection Type
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Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis
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Performance Specifications
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10 fg Anthracene, Ex=250 nm, Em=400 nm* RAMAN single wavelength (H2O) > 500 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime) RAMAN dual wavelength (H2O) > 300 with Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds responsetime)
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Light source
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Xenon Flash Lamp, normal mode 20 W, economy mode 5 W
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Pulse frequency
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296 Hz for single signal mode 74 Hz for spectral mode
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Excitation Monochromator
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Range: 200 nm - 700 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 300 nm
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Emission Monochromator
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Range: 280 nm - 900 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator:concave holographic grating, F/1.6, blaze: 400 nm
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Reference System:
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in-line excitation measurement
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Timetable programing
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up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters
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Spectrum acquisition:
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Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200-400 nm, 10 nm step) Step size: 1-20 nm Spectra storage: All
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Wavelength characteristic
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Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting
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Flow cells
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Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz
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Control and data evaluation
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Agilent ChemStation for LC, Agilent Instant Pilot G4208A or Agilent Control Module G1323B with limited spectral data analysis and printing of spectra
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Communications
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Controller-area network (CAN), GPIB, RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals
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