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Detection type
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Multi-signal fluorescence detector with rapid on-line
scanning capabilities and spectral data analysis
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Performance Specifications
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10 fg Anthracene, Ex=250 nm, Em=400 nm*
RAMAN (H2O) > 200 (FLF rev A)
RAMAN (H2O) > 400 (FLF rev >A)
Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds response time)
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Light source
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Xenon Flash Lamp, normal mode 20 W,
economy mode 5 W
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Pulse frequency
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296 Hz for single signal mode, 74 Hz for spectral mode
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Excitation monochromator
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Range: 200 nm - 700 nm and zero-order
Bandwidth: 20 nm (fixed)
Monochromator:concave holographic grating, F/1.6, blaze: 300
nm
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Emission monochromator
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Range: 280 nm - 900 nm and zero-order
Bandwidth: 20 nm (fixed)
Monochromator:concave holographic grating, F/1.6, blaze: 400
nm
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Reference System
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in-line excitation measurement
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Timetable programming
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up to 4 signal wavelengths, response time, PMT Gain,
baseline behavior (append, free, zero), spectral parameters
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Spectrum acquisition
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Excitation or Emission spectra
Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200-400 nm, 10 nm step)
Step size: 1-20 nm
Spectra storage: All
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Wavelength characteristic
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Repeatability ±0.2 nm
Accuracy ±3 nm setting
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Flow cells
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Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum,
quartz
Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz
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Control and data evaluation
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Agilent ChemStation for LC, Agilent 1100 Control Module with
limited spectral data analysis and printing of spectra
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Analog outputs
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Recorder/integrator: 100 mV or 1 V, output range >102
luminescence units, two outputs
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Communications
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Controller-area network (CAN),
GPIB, RS-232C, LAN, APG
Remote: ready, start, stop and shut-down signals
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Safety and Maintenance
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Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
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GLP Features
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Early Maintenance Feedback (EMF) for continuous tracking of
instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy, using the Raman band of water
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Housing
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All materials recyclable
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Environment
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0 to 40 °C constant temperature at <95% humidity
(non-condensing)
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Dimensions and Weight
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140 mm x 345 mm x 435 mm (5.5 x 13.5 x 17 inches)
(height x width x depth)
25.5 lbs
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