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Thermo Fisher Countess II FL Automated Cell Counter
Concentration
1x10^4-1x10^7 cells/ml
Detection Method
Brightfield
Cell Size
4-60 um (detection), 7-70 um (viability)
- High accuracy eliminates the subjectivity of manual cell counting
- Fast in providing total cell count, viability and average cell size within as little as 10 seconds
- Flexibility in ability to accept up to two EVOS light cubes to cover over twenty fluorescence channels
- Convenient since not cleanup or routine maintenance is required
- Simple user interface to provide quick startup and minimal training
- Easy to transfer data and images to your PC
The Thermo Fisher Countess II FL Automated Cell Counter is a fully automated, 3-channel cell counter and assay platform that uses state-of-the-art optics, and image analysis algorithms to analyze fluorescently labeled cells or trypan blue stained samples in suspension. The Countess II FL features a cell sample range of 1 x 10
4 - 1 x 10 7 and processing time in as little as 10 seconds. The featured digital display shows images of cells and sample data making interpretation convenient and easy. The USB port allows users to save cell count results and images and then transfer them to a PC. The compact design allows for this versatile machine to fit easily into any lab environment. The Countess II FL Cell Counter can accomplish all of the functions that the Countess II Cell Counter is capable of, however adds on some additional features. The Countess II FL can count cells from either disposable Countess Cell Counting Chamber Slides or from glass Countess II FL Reusable Chamber Slides. In addition to the brightfield channel, the Countess II FL can accommodate two interchangeable EVOS fluorescent or specialty light cubes, enabling it to be used for multiple-fluorescence research applications. When equipped with EVOS light cubes, this unit can be used to perform assays for cells in suspension, including simultaneous counts of cells stained with two different fluorescent dyes, GFP and RFP expression, apoptosis, cell viability, and cell cycle assays. All of our equipment is in excellent condition and will be fully tested to perform at factory specifications before being shipped.
The Thermo Fisher Countess II FL Automated Cell Counter includes:
- > Phosphate-buffered Saline Calibration & Countess Test Beads Validation Report
- > 10 Countess Cell Counting Chamber Slides
- > Power Cord
- > Full 180 Day Parts and Labor Warranty
Thermo Fisher Countess II FL Automated Cell Counter Specifications
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Capacity
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One 2-chamber
disposable slide or 2-chamber reusable slide
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Cell Size
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4-60 µm
(detection), 7-60 m (viability)
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Concentration
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1x10^4-1x10^7
cells/mL
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Detection
Method
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Brightfield & 2 user-definable FL channels
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Dimensions (W x D x H)
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9 x 5.5 x 9 in (228.6 x 139.7 x 228.6 mm)
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Display
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7 in capacitive touch screen
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Field
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2.15 mm x
1.62 mm (3.48 mm2)
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Memory
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USB Stick
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Electrical
Requirements
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100-240 VAC, 0.58A Max
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Weight
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8 lbs.
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Thermo Fisher Countess II FL Automated Cell Counter
 Click Here to View the Technical Specs for the Thermo Fisher Countess II FL Cell Counter
- Verify machine software initialization to ensure complete and accurate startup
- Examine display screen for cracks, dead pixels, or other damage that may affect visibility
- Test touch screen functionality, confirming sensitivity and accurate response
- Inspect slide port for proper alignment and any visible signs of wear or obstruction
- Examine internal wiring for secure connections and any signs of damage or wear
- Clean internal casing to maintain optimal equipment hygiene and prevent dust buildup
- Prepare a test sample using a precise 10µL mixture of cell suspension fluid and Trypan Blue stain
- Conduct Phosphate Buffered Saline calibration and validate with Countess test beads, if applicable
- Confirm concentration accuracy of test beads, ensuring results align with specified standards
- Verify zoom, brightness, and focus controls are fully functional for optimal image clarity
- Test USB port functionality, saving test results and images to verify data transfer
- Inspect packaging of disposable chamber slides for any tears, punctures, or contamination
- Verify power supply output for correct voltage delivery to all components
- Compile a comprehensive calibration report, detailing all verification and validation results
Technician Inspection and Recertification Checklist: Cell Counters
The Following Checklist Is to Be Performed on All Incoming and Outgoing Equipment
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Task
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Completed?
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Remarks?
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Verify machine software initialization
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Completed
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Inspect display screen for cracks or damage
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Completed
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Confirm touch screen functionality
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Completed
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Inspect slide port for alignment and wear
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Completed
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Check internal wiring for secure connections and damage
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Completed
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Clean internal case for dust prevention
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Completed
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Prepare 10µL test sample with cell suspension and Trypan Blue
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Completed
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Conduct PBS calibration and Countess test beads validation
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Completed
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Confirm test bead concentration accuracy
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Completed
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Verify zoom, brightness, and focus functionality
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Completed
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Test USB port, save test results and images
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Completed
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Inspect disposable chamber slide packaging for damage
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Completed
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Verify power supply output voltage
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Completed
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Prepare a detailed calibration report
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Completed
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To View More of Our Recertification Protocol Click Here
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