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GE AKTA Micro FPLC System
Flow Rate Range 0.001 to 2 ml/min
Maximum Operating Pressure 35 MPa (350 bar, 5075 psi)
Wavelength Range 190 to 700 nm
- High-performance purification and characterization using microbore to analytical scale columns, all controlled by UNICORN software
- High sample recovery and stability as the complete flow path is manufactured from inert and biocompatible materials
- Optimized resolution by connection of columns directly to the UV cell and by minimized dead volumes in the tubing, valve, and flow cells
- Novel pump design for flexible flow and pressure range enabling high, as well as low pressure separations
- Fast and reproducible purity analysis by gel filtration in method development and drug target characterization
- Absolute characterization of biomolecules in solution and on-line molecular weight analysis by the addition of light scattering equipment
The GE AKTA Micro FPLC System is a highly efficient and versatile chromatography system designed for purifying proteins at micro-scale quantities, emphasizing on maximizing sample recovery and purity in research and development applications. This compact system is ideal for applications requiring precise control and flexibility, such as the purification of monoclonal antibodies, proteins, and nucleotides. The AKTA Micro FPLC's small footprint makes it an excellent choice for laboratories with limited space, while its user-friendly software ensures straightforward setup and operation for both experienced users and newcomers to chromatography. With its capability to handle small sample volumes with high precision, the AKTA Micro FPLC System is an essential tool for biochemists and molecular biologists focusing on the detailed study of biomolecules.
The GE AKTA Micro FPLC System is not only compact and user-friendly but also boasts advanced specifications tailored for precise and efficient chromatographic separations. The system is equipped with a P-905 Pump, offering a wide flow rate range from as low as 0.001 ml/min up to 2 ml/min, accommodating both isocratic and gradient modes, ensuring precise control over your purification process. With a flow rate accuracy of ± 2% or 2 µl/min (whichever is greater) and reproducibility with an RSD of less than 0.5%, the P-905 pump exemplifies reliability and precision, even at low flow rates. Its programmable pressure limits and internal volume of less than 600 µl/pump module make it versatile for various applications, supporting up to 35 MPa (5075 psi) pressure ranges.
The UV-900 Monitor enhances the system's capabilities with a broad wavelength range from 190 to 700 nm, allowing for the detection of multiple absorbances simultaneously, with exceptional accuracy and reproducibility. Its sensitivity and stability ensure accurate monitoring of your sample's purity throughout the process. Incorporating the pH/C-900 Monitor, the system can measure conductivity across a wide range from 1 µS/cm to 999.9 mS/cm, ensuring precise assessment of ionic content in your samples, critical for quality control and consistency in protein purification. The Frac-950 Fraction Collector rounds out this powerful system, designed for flexible fraction collection with a flow rate range of 0.001 to 100 ml/min. Its ability to handle a wide pH stability range ensures compatibility with a variety of samples, while its precise fraction size control and no spillage range make it an indispensable tool for collecting your purified compounds.
All of our equipment is fully tested to perform at factory specifications before being shipped.
The GE AKTA Micro FPLC System includes:
- > Box-900
- > pH/C 900 Monitor
- > UV-900 Monitor
- > P-905 Pump
- > INV-917 Valve
- > M-925 Mixer w/ 0.2 ml Mixing Chamber
- > Conductivity Flow Cell
- > CU-950 Communication Module
- > Frac-950 Fraction Collector with Tube Rack
- > Dell Windows 7 Datastation with Installed UNICORN Version 5.31 Software
- > Installation Calibration Report
- > Communication Cables
- > Tubing, Power Cords
- > Full 180 Day Parts and Labor Warranty
GE AKTA Micro FPLC System Specifications
P-905 Pump Specifications
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Flow Rate Range
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Isocratic mode: 0.001 to 2 ml/min in steps of 1 ul/min
Gradient mode: 0.04 to 2 ml/min in steps of 1 ul/min
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Flow Rate Accuracy
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± 2% or 2 ul/min, whichever is greater with compression compensation activated
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Flow Rate Reproducibility
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> 0.04 ml/min, RSD <0.5%
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Increment
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0.001 ml/min
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Pressure Range
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0 to 35 MPa (350 bar, 5075 psi)
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Pressure Limits
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Programmable upper and lower
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Internal Volume
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< 600 ul/pump module
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Viscosity
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Maximum: 3cP
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Peak Tubing Dimensions
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0.15 mm i.d.
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UV-900 Monitor Specifications
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Wavelength Range
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190 to 700 nm in steps of 1 nm, three wavelengths simultaneously
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Bandwidth
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4 nm
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Wavelength Accuaracy
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± 2 nm
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Wavelength Reproducibility
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± 0.01 nm
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Linearity
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< 2% deviation up to 2 AU at 260 nm, with uracil at pH 2
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Noise at 230 nm
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< 6 x 10
-5 AU with 3 mm cell
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Drift at 254 nm
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< 2 x 10
-4 AU/h
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Flow Cell Maximum Flow Rate
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2 ml/min
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Flow Cell Maximum Pressure
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2 MPa (20 bar, 290 psi)
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Flow Cell Path Length
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3 mm
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Flow Cell Internal Volume
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0.7 ul
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pH/C-900 Monitor Specifications
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Conductivity Range
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1 uS/cm to 999.9 mS/cm
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Deviation from Theoretical Conductivity
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Maximum ± 2% of full-scale calibration range or ± 10 uS/cm, whichever is greater in range
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Noise
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Maximum ± 0.5% of full-scale calibration range
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Flow Cell Maximum Flow Rate
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2 ml/min
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Flow Cell Maximum Pressure
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5 MPa (50 bar, 725 psi)
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Flow Cell Internal Volume
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Max 5 cP
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Frac-950 Fraction Collector Specifications
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Flow Rate Range
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0.001 to 100 ml/min
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pH Stability Range
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1 to 3, 1 to 14 (> 1 day exposure)
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Fraction Size
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Volume mode: 0.04 to 99999.99 ml
Time Mode: 0.04 to 99999.99 ml
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No Spillage Range
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Dropsync range: 0 to 3 ml/min
Accumulator range: 15 to 100 ml/min
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Computer Specifications
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Model
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Dell Optiplex 7010 Desktop PC
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Processor
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Intel Core i5-6500 Processor 3.6GHz
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RAM
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16GB
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Hard Drive
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250GB Internal SSD
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Operating System
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Windows 7 Professional, 64-bit
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Every product we offer undergoes a rigorous inspection process to verify that all manufacturer specifications are met. All of the electronics and boards are tested for proper function, and any necessary preventative maintenance is performed alongside repairs by our industry trained technicians. Our primary goal is to offer products that are as close to new as possible at an affordable price.
All Lab Equipment is Fully Recertified and Tested
Most Pieces Ship within 1 to 3 Business Days
All Products are Fully Warrantied
Trained Customer and Technical Support
Industry Leading Overall Customer Satisfaction
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