Waters 2695 HPLC System with 2487 UV-Vis Detector

Number of solvents

One to four

Solvent conditioning

Vacuum degas, two (2) operating modes, four (4) chambers, ?<500 uL internal volume per chamber> <500uL interval volume per chamber

Programmable flow rate range

0.000 and 0.010 to 10.000 mL/min in 0.001-mL/min. increments

Typical operating flow rate range

0.050 to 5.000 mL/min in 0.001-mL/min increments

Compressibility compensation

Automatic and continuous

Effective system delay volume

<650 L, independent of backpressure @ 1 mL/min

Plunger seal wash

Integra, active, programmable

Gradient profiles

Eleven (11) gradient curves [including linear, step (2 curves), concave (4 curves), and convex (4 curves)]

Dry prime/wet prime

Automatic, front panel control

Flow ramping

Time (0.01 to 30.00 min in 0.01-min increments) to reach maximum flow rate

Maximum operating pressure

5000 psi (345 bar) (0.010 to 3.000 mL/min) Programmable upper and lower limits. Pressure fall-off at >3.000 mL/min.

Pressure ripple

?2.5% (1 mL/min, degassed methanol, at 1700 psi backpressure)

Composition accuracy

0.5% absolute, independent of backpressure (Proportioning Valve Pair Test [degassed methanol:methanol/propylparaben, 2 mL/min, 254 nm])

Composition precision

?0.15% RSD or ?0.02 min SD, whichever is greater, based on retention time. (Degassed methanol:water 60:40 Dial-a-Mix, 1 mL/min, six replicates, phenone mix, 254 nm.)

Flow precision

?0.075% RSD or ?0.02 min SD, based on retention time (N = 6) or volumetric measures (0.200 to 5.000 mL/min), isocratic premix

Flow accuracy

1% or 10 L/min, whichever is greater, (0.200 to 5.000 mL/ min), degassed methanol, at 600 psi backpressure